Primers for TLR5 (R392X) were T5?0, forward, and T5?1, reverse
Primers for TLR5 (R392X) had been T5?0, forward, and T5?1, reverse, 5-GAGATCCAAGGTCTGTAATTTTTCCAGG-3 . End-point analysis was performed by high-resolution melting curve evaluation employing LightCycler 480 software program (Roche). In vitro…