HjH strain. For the biofilm cells, the -galactosidase activity was measured in the dispersed cells. Signifies and SD in -galactosidase activity from triplicate experiments are shown. *, P 0.01.aac.asm.orgAntimicrobial Agents and ChemotherapyC-di-GMP Regulates Resistance in P. aeruginosaTABLE 2 Proteins whose abundance in P. aeruginosa PAO1 increased substantially in circumstances of low intracellular levels of c-di-GMPPA no. PA2452 PA1092 PA3531 PA4777 PA1596 PA2821 PA4386 PA3126 PA4228 PA1039 PA3552 PA4942 PA1534 PA4670 PA4710 PA4227 PA4224 PA3158 PA0427 PA0018 PA1803 PA3135 PA4231 PA3114 PA3831 PA3159 PA5054 PA4225 PA0426 PA1288 PA4749 PA5213 PA3478 PA2086 PA4336 PA4595 PA4476 PA2290 PA5237 PA2302 PA4307 PA3707 PA4588 PAaGene fliC bfrB pmrB htpG gstA groES ibpA pchD ychJ arnB hflK recR prs phuR pchR pchG wbpB oprM fmt lon pchA truA pepA wbpA hslU pchF mexB ompP1 glmM gcvP1 rhlB ephx yjjK gcd yigC ambE pctC wspB gdhA pchEDescription of product Enterochelin esterase Flagellin sort B Bacterioferritin PmrB: two-component regulator method signal sensor kinase PmrB Heat shock protein 90 Glutathione S-transferase Cochaperonin GroES Heat shock protein Hsp20 Pyochelin biosynthesis protein PchD Hypothetical protein O1Q_07577 UDP-4-amino-4-deoxy-L-arabinose xoglutarate aminotransferase Protease subunit HflK Recombination protein RecR Ribose-phosphate pyrophosphokinase Heme/hemoglobin uptake outer membrane receptor PhuR Transcriptional regulator PchR Pyochelin biosynthetic protein PchG UDP-2-acetamido-2-deoxy-D-glucuronic acid 3dehydrogenase, WbpB Big intrinsic several antibiotic resistance efflux outer membrane protein OprM Bifunctional UDP-glucuronic acid decarboxylase/UDP-4amino-4-deoxy-L-arabinose formyltransferase Putative ATP-dependent protease Putative transcriptional regulator Salicylate biosynthesis isochorismate synthase tRNA pseudouridine synthase A Multifunctional aminopeptidase A UDP-glucose/GDP-mannose dehydrogenase ATP-dependent protease ATP-binding subunit HslU pchF gene product RND multidrug efflux transporter MexB Putative outer membrane protein Phosphoglucosamine mutase Glycine dehydrogenase Rhamnosyltransferase chain B Epoxide hydrolase Hypothetical protein O1Q_03368 Putative ABC transporter ATP-binding protein Hypothetical protein O1Q_04078 Glucose dehydrogenase 3-Octaprenyl-4-hydroxybenzoate carboxy-lyase Protein AmbE pctC gene item Hypothetical protein O1Q_02948 Glutamate dehydrogenase pchE gene productPeptides (95 ) 43 146 33 two 30 5 2 15 14 two 4 6 2 43 20 3 16 10 13 3 23 2 four 3 42 17 six 24 six eight five three 2 3 9 15 six 9 three 20 20 2 30Coverage (95 ) 53.Formula of 2-Bromo-6-hydroxybenzaldehyde 42 54.3 34.18 five.451 22.four 20.91 45.36 24.16 13.71 15.29 13.09 17 21.21 56.55 25.52 15.54 30.37 22.78 32.16 eight.006 25.28 11.11 12.39 17.54 38.59 31.42 13.87 16.8 5.067 24.76 eight.Buy4-Chloro-5-methoxypyridin-2-amine 09 3.PMID:24367939 967 7.512 19.33 23.32 24.37 five.956 9.34 six.967 10.34 31.65 14.79 41.57 30.Ratio (115:114) 99.08 99.08 22.28 11.91 ten.57 10.28 9.20 eight.95 eight.24 7.94 7.38 7.31 7.31 7.11 six.55 six.31 six.25 6.08 5.40 five.15 4.79 4.61 4.57 three.98 three.80 three.66 3.44 three.25 three.25 3.19 3.16 two.91 2.86 2.75 two.73 two.68 two.51 2.42 two.42 two.27 two.11 2.07 two.05 2.P a (115:114) five.22E?3 three.19E?two 2.82E?four three.28E?2 1.58E?2 3.68E?two 3.15E?2 1.20E?2 3.35E?3 3.79E?two 2.85E?two two.24E?3 three.72E?two 8.95E?five 1.57E?four two.34E?2 9.10E?3 three.03E?two 1.56E?3 2.80E?2 1.21E?4 three.86E?two 4.12E?two 3.39E?2 2.66E?two five.69E?three four.85E?2 five.80E?six 3.05E?3 two.64E?two 3.36E?two eight.00E?three 3.40E?3 three.59E?2 3.78E?three 9.43E?4 9.67E?three 3.72E?2 four.15E?two 1.66E?2 2.50E?three 3.07E?two three.19E?2 1.85E?Significance was defined as possessing a 115:114 abunda.