Eat-inactivated FBS (hiFBS; Thermo Scientific, Waltham, MA). Cells had been enumerated at
Eat-inactivated FBS (hiFBS; Thermo Scientific, Waltham, MA). Cells were enumerated at a 1:1 dilution with 0.2 trypan blue stain (Life Technologies, Grand Island, NY) applying a Cellometer Auto T4 Cell…